Brief Reports
11 August 2010
Vol. 1 No. 2 (2010)
https://doi.org/10.4081/pb.2010.e14

A rapid method for isolation of low-molecular-weight RNA from Arabidopsis using low salt concentration buffer

Publisher's note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Low molecular RNA, Arabidopsis
12192
Views
564
Downloads
10731
HTML

Authors

Han Cheng
forcheng@gmail.com
Jing Gao
Zewei An
Huasun Huang
We have developed a rapid extraction method using low salt concentration buffer for the isolation of low-molecular-weight RNA from Arabidopsis tissues. The method was quick and efficient, and the small scale extraction process took no more than 1 hour, while yield and RNA quality were comparable with those of previously reported. The LMW RNA isolated using this method was high quality, abundant in small RNA and free of high molecular weight RNA. This method can be used to extract low-molecular-weight RNA for the purpose of small RNA cloning and detection, and library construction.

Altmetrics

Downloads

Download data is not yet available.

Citations

Crossref
Scopus
Google Scholar
Europe PMC

Supporting Agencies

Rubber Researchi Insititute, Chinese Academy of Tropical Agricultural Science

How to Cite



A rapid method for isolation of low-molecular-weight RNA from Arabidopsis using low salt concentration buffer. (2010). International Journal of Plant Biology, 1(2), e14. https://doi.org/10.4081/pb.2010.e14