Articoli originali
20 marzo 2025
V. 13 (2025)
Profilo di resistenza degli isolati di Candida spp. da emocoltura identificati mediante reazione a catena delle polimerasi real-time: 2022-2023
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All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
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Background: descrivere i pattern di suscettibilità ai farmaci antifungini degli isolati di Candida spp. identificati mediante reazione a catena delle polimerasi real-time da emocolture positive prelevate da pazienti ricoverati presso l'Azienda Ospedaliero-Universitaria SS. Antonio e Biagio e Cesare Arrigo di Alessandria nel periodo 2022-2023.
Materiali e Metodi: sono stati inclusi nello studio tutti i pazienti dei quali erano disponibili dati clinici, che sono stati sottoposti a prelievo per emocoltura risultata positiva soltanto per lieviti. Tutte le emocolture incluse sono state processate sia mediante reazione a catena delle polimerasi real-time che mediante esame colturale.
Risultati: nella nostra casistica di 67 emocolture positive soltanto per lieviti, tutti gli isolati sono risultati sensibili alle echinocandine e all'amfotericina B. Otto isolati su 19 (42%) di Candida parapsilosis e 2/14 (14.3%) di Candida glabrata sono risultati resistenti al fluconazolo.
Conclusioni: la rapida identificazione dell'isolato fungino mediante reazione a catena delle polimerasi real-time associata alla conoscenza dei pattern locali di suscettibilità ai farmaci può essere di aiuto nella scelta della migliore terapia antifungina, in attesa dell’antimicogramma.
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Battistolo J, Glampedakis E, Damonti L, et al. Increasing morbidity and mortality of candidemia over one decade in a Swiss university hospital. Mycoses 2021;64:1512-20. DOI: https://doi.org/10.1111/myc.13376
Muderris T, Kaya S, Ormen B, et al. Mortality and risk factor analysis for Candida blood stream infection: a three-year retrospective study. J Mycol Med 2020;30:101008. DOI: https://doi.org/10.1016/j.mycmed.2020.101008
Poissy J, Damonti L, Bignon A, et al. Risk factors for candidemia: a prospective matched case-control study. Crit Care 2020;24:109. DOI: https://doi.org/10.1186/s13054-020-2766-1
Zhang MK, Rao ZG, Ma T, et al. Appropriate empirical antifungal therapy is associated with a reduced mortality rate in intensive care unit patients with invasive fungal infection: A real-world retrospective study based on the MIMIC-IV database. Front Med (Lausanne) 2022;9:952611. DOI: https://doi.org/10.3389/fmed.2022.952611
Pfaller MA, Carroll KC, et al. Manual of clinical microbiology. 13th ed. ASM Press; Washington, DC, USA; 2023.
Spanu T, Posteraro B, Fiori B, et al. Direct MALDI-TOF mass spectrometry assay of blood culture broths for rapid identification of Candida species causing bloodstream infections: an observational study in two large microbiology laboratories. J Clin Microbiol 2012;50:176-9. DOI: https://doi.org/10.1128/JCM.05742-11
Posteraro B, Torelli R, De Carolis E, et al. Update on the laboratory diagnosis of invasive fungal infections. Mediterr J Hematol Infect Dis 2011;3:e2011002. DOI: https://doi.org/10.4084/mjhid.2011.002
Gaibani P, Rossini G, Ambretti S, et al. Blood culture systems: rapid detection-how and why? Int J Antimicrob Agents 2009;34:S13-5. DOI: https://doi.org/10.1016/S0924-8579(09)70559-X
Paolucci M, Stanzani M, Melchionda F, et al. Routine use of a real-time polymerase chain reaction method for detection of bloodstream infections in neutropaenic patients. Diagn Microbiol Infect Dis 2013;75:130-4. DOI: https://doi.org/10.1016/j.diagmicrobio.2012.10.012
Mauri C, Consonni A, Briozzo E, et al. Microbiological assessment of the FilmArray Blood culture identification 2 panel: potential impact in critically ill patients. Antibiotics (Basel) 2023;12:1247. DOI: https://doi.org/10.3390/antibiotics12081247
Monti G, Landoni G, Taddeo D, et al. Clinical aspects of sepsis: an overview. Methods Mol Biol 2015;1237:17-33. DOI: https://doi.org/10.1007/978-1-4939-1776-1_3
Yapar N. Epidemiology and risk factors for invasive candidiasis. Ther Clin Risk Manag 2014;10:95-105. DOI: https://doi.org/10.2147/TCRM.S40160
Leli C, Gotta F, Ferrara L, et al. Bayesian estimation of post-test probability of Candida glabrata fungemia by means of serum creatinine. New Microbiol 2022;45:324-30.
BioMérieux Diagnostics. BioFire FilmArray Blood Culture Identification (BCID) panel. Instruction booklet. RFIT-ASY-0126 RFIT-ASY-0127. Available from: https://www.biofiredx.com/support/documents/#toggle-id-4
MERLIN a Bruker Company© MERLIN Diagnostika GmbH. MICRONAUT-AM. Provided by the manufacturer. 2018. Available from: https://www.bruker.com/pl/products-and-solutions/microbiology-and-diagnostics/antimicrobial-susceptibility-testing/micronaut-am.html
Opentrons. Ot-2 robot. 2023. Available from: https://shop.opentrons.com/ot-2-robot/
The European Committee on Antimicrobial Susceptibility Testing. Breakpoint tables for interpretation of MICs for antifungal agents, version 10.0. 2020. Available from: http://www.eucast.org/astoffungi/clinicalbreakpointsforantifungals/
Pfaller MA, Messer SA, Diekema DJ, et al. Use of micafungin as a surrogate marker to predict susceptibility and resistance to caspofungin among 3,764 clinical isolates of Candida by use of CLSI methods and interpretive criteria. J Clin Microbiol 2014;52:108-14. DOI: https://doi.org/10.1128/JCM.02481-13
Pfaller MA, Diekema DJ, Jones RN, Castanheira M. Use of anidulafungin as a surrogate marker to predict susceptibility and resistance to caspofungin among 4,290 clinical isolates of Candida by using CLSI methods and interpretive criteria. J Clin Microbiol 2014;52:3223-9. DOI: https://doi.org/10.1128/JCM.00782-14
Mareković I, Pleško S, Rezo Vranješ V, et al. Epidemiology of candidemia: three-year results from a Croatian tertiary care hospital. J Fungi (Basel) 2021;7:267. DOI: https://doi.org/10.3390/jof7040267
Bourassa-Blanchette S, Biesheuvel MM, Lam JC, et al. Incidence, susceptibility and outcomes of candidemia in adults living in Calgary, Alberta, Canada (2010-2018). BMC Infect Dis 2023;23:100. DOI: https://doi.org/10.1186/s12879-023-08050-0
Pfaller MA, Diekema DJ, Jones RN, et al. Trends in antifungal susceptibility of Candida spp. isolated from pediatric and adult patients with bloodstream infections: SENTRY Antimicrobial Surveillance Program, 1997 to 2000. J Clin Microbiol 2002;40:852-6. DOI: https://doi.org/10.1128/JCM.40.3.852-856.2002
Chesdachai S, Baddour LM, Sohail MR, et al. Candidemia in patients with cardiovascular implantable electronic devices: uncertainty in management based on current international guidelines. Open Forum Infect Dis 2023;10:ofad318. DOI: https://doi.org/10.1093/ofid/ofad318
Mahmoud A, Khalid AB, Ehle A, et al. Does heart failure mask Candida tricuspid endocarditis? Cureus 2023;15:e38951. DOI: https://doi.org/10.7759/cureus.38951
Giacobbe DR, Salsano A, Del Puente F, et al. Risk factors for candidemia after open heart surgery: results from a multicenter case-control study. Open Forum Infect Dis 2020;7:ofaa233. DOI: https://doi.org/10.1093/ofid/ofaa233
Alhatmi H, Almansour S, Abanamy R, et al. Clinical characteristics and outcome of candidemia: experience from a tertiary referral center in Saudi Arabia. Saudi J Med Med Sci 2022;10:125-30. DOI: https://doi.org/10.4103/sjmms.sjmms_625_21
Koehler P, Stecher M, Cornely OA, et al. Morbidity and mortality of candidaemia in Europe: an epidemiologic meta-analysis. Clin Microbiol Infect 2019;25:1200-12. DOI: https://doi.org/10.1016/j.cmi.2019.04.024
Come citare
Profilo di resistenza degli isolati di Candida spp. da emocoltura identificati mediante reazione a catena delle polimerasi real-time: 2022-2023. (2025). Working Paper of Public Health, 13. https://doi.org/10.4081/wpph.2025.10062
Copyright (c) 2025 Christian Leli, Lidia Ferrara, Paolo Bottino, Luigi Di Matteo, Marco Leone, Camilla Furiani, Elisabetta Scomparin, Annalisa Roveta, Antonio Maconi, Andrea Rocchetti, Daria Vay, Franca Gotta, Elisa Cornaglia
Questo volume è pubblicato con la licenza Creative Commons Attribuzione - Non commerciale 4.0 Internazionale.
