Original Article(s)
18 December 2023
Vol. 11 (2023)
https://doi.org/10.4081/wpph.2023.9643

Analysis of lymphocyte subsets in COVID-19 patients: a retrospective observational study

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COVID-19, Lymphocyte subset, Flow cytometry
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Authors

Maria Matilde Ciriello
SC Laboratorio Analisi, Azienda Ospedaliera SS. Antonio e Biagio e Cesare Arrigo, Alessandria, Italy.
Nicoletta Tommasi
SC Laboratorio Analisi, Azienda Ospedaliera SS. Antonio e Biagio e Cesare Arrigo, Alessandria; Dipartimento di Scienze e Innovazione Tecnologica, Università del Piemonte Orientale, Alessandria, Italy.
Costanza Massarino
costanza.massarino@ospedale.al.it
https://orcid.org/0009-0002-2744-5301
SSD Laboratori di Ricerca – Dipartimento Attività Integrate Ricerca Innovazione, Azienda Ospedaliera SS. Antonio e Biagio e Cesare Arrigo, Alessandria, Italy.
Antonella Cassinari
https://orcid.org/0009-0001-7954-0055
SC Infrastruttura Ricerca Formazione Innovazione – Dipartimento Attività Integrate Ricerca Innovazione, Azienda Ospedaliera SS. Antonio e Biagio e Cesare Arrigo, Alessandria, Italy.
Thea Bensi
https://orcid.org/0009-0008-3782-5213
SC Laboratorio Analisi, Azienda Ospedaliera SS. Antonio e Biagio e Cesare Arrigo, Alessandria, Italy.
Raffaella Doglio
https://orcid.org/0009-0004-7506-7055
SC Laboratorio Analisi, Azienda Ospedaliera SS. Antonio e Biagio e Cesare Arrigo, Alessandria, Italy.
Annalisa Roveta
https://orcid.org/0000-0003-2484-0963
SSD Laboratori di Ricerca – Dipartimento Attività Integrate Ricerca Innovazione, Azienda Ospedaliera SS. Antonio e Biagio e Cesare Arrigo, Alessandria, Italy.
Antonio Maconi
Dipartimento Attività Integrate Ricerca Innovazione, Azienda Ospedaliera SS. Antonio e Biagio e Cesare Arrigo, Alessandria, Italy.

In this retrospective study, we analyzed the possible predictive changes in lymphocyte subsets of Coronavirus Disease 19 (COVID-19) hospitalized patients. We enrolled 107 COVID-19 patients older than 18 years of age, admitted to Alessandria Hospital with a confirmed diagnosis of SARS-CoV-2 infection by Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR), who performed the analysis of lymphocyte subsets, between 2020 March 01 and 2021 May 31. Patients have been split up into two groups, based on clinical manifestations: group 1 non-severe disease (n=44) and group 2 severe disease (n=63), according to the World Health Organization (WHO) interim guidance. For the comparison between the two groups of patients, statistical significance was tested with the Mann-Whitney and the Chi-Square test. In the group of patients with severe disease, the blood cell analysis showed a significant reduction in the counts of total lymphocytes, absolute CD3 lymphocytes, and CD8 lymphocytes, as well as a significant reduction in monocyte percent and a very significant increase in neutrophil counts. From the results obtained, it can be stated that lymphocytopenia is associated with the progression of the disease and increased mortality.

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How to Cite



Analysis of lymphocyte subsets in COVID-19 patients: a retrospective observational study. (2023). Working Paper of Public Health, 11(1). https://doi.org/10.4081/wpph.2023.9643
Copyright (c) 2023 Maria Matilde Ciriello, Nicoletta Tommasi, Costanza Massarino, Antonella Cassinari, Thea Bensi, Raffaella Doglio, Annalisa Roveta, Antonio Maconi Creative Commons License

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