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9 gennaio 2024

Impiego di dati clinici e di laboratorio per stimare la probabilità di diarrea ad eziologia batterica diagnosticata mediante multiplex Real-Time PCR su campioni di feci in una popolazione pediatrica

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Obiettivi: in questo report abbiamo stimato la probabilità di un risultato positivo per i più comuni agenti causali batterici di diarrea, quali Campylobacter spp., Salmonella spp., Shigella/Escherichia coli enteroinvasiva, Yersinia enterocolitica od Escherichia coli produttori di tossine Shiga, identificati mediante una multiplex Real-Time PCR su campioni di feci in una popolazione pediatrica valutata presso la Struttura Complessa di Pediatria e Pronto Soccorso Pediatrico dell’Azienda Ospedaliera SS. Antonio e Biagio e Cesare Arrigo di Alessandria, nel periodo aprile 2022 - luglio 2023. Metodi: sono stati analizzati i dati clinici inseriti dal Pediatra sul modulo di richiesta di esame molecolare su campione di feci prelevate da pazienti con diarrea a sospetta eziologia infettiva contemporaneamente all’emocromo completo ed alla proteina C reattiva. Risultati: nella nostra casistica, la presenza di sangue/muco nelle feci associata ad un elevato valore di proteina C reattiva sono risultati indipendentemente associati ad una probabilità più elevata di risultato positivo mediante metodo molecolare per i batteri descritti sopra. Conclusioni: i risultati di questo lavoro possono essere di aiuto per stimare la probabilità di diarrea ad eziologia batterica in un paziente pediatrico in strutture ospedaliere senza la disponibilità di un test molecolare.

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Impiego di dati clinici e di laboratorio per stimare la probabilità di diarrea ad eziologia batterica diagnosticata mediante multiplex Real-Time PCR su campioni di feci in una popolazione pediatrica. (2024). Working Paper of Public Health, 11(1). https://doi.org/10.4081/wpph.2023.9842